Hotspot

rps12-trnV-GAC

IRb · 98961-100451 · 1491 bp

Back to Omphalogramma

Pi 0.00335
MarkerSeek score 62.80
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1491
variable_sites
13
pi
0.00335
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GGATAACCAGGCTCGAACTG 45.0 55.0 57.7 58.1 1594-1629 bp 1.0 79.0
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1575-1610 bp 1.0 78.2
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1579-1614 bp 1.0 78.1
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1574-1609 bp 1.0 78.1
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1577-1612 bp 1.0 77.2