Hotspot

psbE-petL

LSC · 65094-66317 · 1224 bp

Back to Omphalogramma

Pi 0.0171
MarkerSeek score 63.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1224
variable_sites
53
pi
0.0171
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC CCGAAATAACTAGTTATCGTAGGC 50.0 41.7 57.8 57.1 1271-1299 bp 1.0 45.9
psbE-petL_p2 CAACCCGCAATGAATAGGGA CCGAAATAACTAGTTATCGTAGGC 50.0 41.7 57.9 57.1 1348-1376 bp 1.0 45.7
psbE-petL_p3 ACGAATAACCAACCCGCAAT CCGAAATAACTAGTTATCGTAGGC 45.0 41.7 58.2 57.1 1357-1385 bp 1.0 45.5
psbE-petL_p4 TGCTGACGAATAACCAACCC CCGAAATAACTAGTTATCGTAGGC 50.0 41.7 58.2 57.1 1362-1390 bp 1.0 45.5
psbE-petL_p5 CGAATAACCAACCCGCAATG CCGAAATAACTAGTTATCGTAGGC 50.0 41.7 57.8 57.1 1356-1384 bp 1.0 45.5