Hotspot

rps4-trnT-UGU

LSC · 46105-46450 · 346 bp

Back to Omphalogramma

Pi 0.0262

MarkerSeek score 68.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 21
pi: 0.0262
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGGCGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	454-460 bp	1.0	81.5
rps4-trnT-UGU_p2	GTAAGTCCGGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	470-476 bp	1.0	81.1
rps4-trnT-UGU_p3	CTCCCAGGCGACGTATTTTT	ATTCTATGAGATGCGCCTGC	50.0	50.0	58.3	58.2	478-484 bp	1.0	80.8
rps4-trnT-UGU_p4	CAGCTCGGGGCCTTTTATTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	490-496 bp	1.0	80.6
rps4-trnT-UGU_p5	GCTCGGGGCCTTTTATTAGT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	488-494 bp	1.0	80.6