Hotspot

trnS-UGA-psbZ

LSC · 35728-36076 · 349 bp

Back to Omphalogramma

Pi 0.0203

MarkerSeek score 59.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 16
pi: 0.0203
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ATACAACGGGTACGCCAATC	55.0	50.0	58.0	58.1	442-449 bp	1.0	84.2
trnS-UGA-psbZ_p2	GTTTTCAAGACCGGGGCTAT	ATACAACGGGTACGCCAATC	50.0	50.0	57.9	58.1	462-469 bp	1.0	83.2
trnS-UGA-psbZ_p3	CAACCACTCAGCCATCTCTC	AAATACAACGGGTACGCCAA	55.0	45.0	58.0	57.8	444-451 bp	1.0	83.2
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.0	57.7	461-468 bp	1.0	82.3
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	AAATACAACGGGTACGCCAA	50.0	45.0	57.9	57.8	464-471 bp	1.0	82.2