Hotspot

trnT-GGU-psbD

LSC · 31790-32975 · 1186 bp

Back to Omphalogramma

Pi 0.0158

MarkerSeek score 63.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 47
pi: 0.0158
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	AGCAATAAACCAGACCAGCC	50.0	50.0	58.3	58.2	1335-1356 bp	1.0	80.0
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GCAATAAACCAGACCAGCCT	50.0	50.0	58.3	58.2	1334-1355 bp	1.0	80.0
trnT-GGU-psbD_p3	CCCCTTTAACTCAGCGGTAG	AGCAATAAACCAGACCAGCC	55.0	50.0	57.7	58.2	1371-1392 bp	1.0	79.7
trnT-GGU-psbD_p4	CCCCTTTAACTCAGCGGTAG	GCAATAAACCAGACCAGCCT	55.0	50.0	57.7	58.2	1370-1391 bp	1.0	79.7
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1314-1335 bp	1.0	79.4