Hotspot

trnC-GCA-petN

LSC · 28025-28486 · 462 bp

Back to Omphalogramma

Pi 0.0131

MarkerSeek score 62.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 17
pi: 0.0131
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	504-513 bp	1.0	79.0
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	565-574 bp	1.0	77.0
trnC-GCA-petN_p3	CGGGGGACTGCAAATCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.7	58.2	530-539 bp	1.0	76.7
trnC-GCA-petN_p4	CGGGGGACTGCAAATCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.7	57.6	591-600 bp	1.0	74.7
trnC-GCA-petN_p5	CGGGGGACTGCAAATCTTT	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.0	58.2	530-539 bp	1.0	74.4