Hotspot

atpF-atpH

LSC · 13121-13501 · 381 bp

Back to Omphalogramma

Pi 0.019

MarkerSeek score 69.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 17
pi: 0.019
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGAGGCAGAGGGAAAAATCC	50.0	50.0	57.9	57.5	568-571 bp	1.0	78.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	570-573 bp	1.0	78.7
atpF-atpH_p3	CCAGTAGCCCAAAGAAACGA	TGAGGCAGAGGGAAAAATCC	50.0	50.0	57.8	57.5	541-544 bp	1.0	78.4
atpF-atpH_p4	CCAGTAGCCCAAAGAAACGA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.8	57.5	543-546 bp	1.0	78.4
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATCCGAGG	50.0	55.0	57.9	58.6	564-567 bp	1.0	77.9