Hotspot

trnS-CGA-trnR-UCU

LSC · 9826-10084 · 259 bp

Back to Omphalogramma

Pi 0.0225

MarkerSeek score 72.20

Primer available yes

Label trnS-CGA-trnR-UCU

Feature Metadata

label_name: trnS-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 15
pi: 0.0225
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGGAATACATTGCGTCCAA	50.0	45.0	58.0	57.5	385-400 bp	1.0	81.5
trnS-CGA-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGGAATACATTGCGTCCAA	55.0	45.0	58.1	57.5	388-403 bp	1.0	81.2
trnS-CGA-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	ATGGGAATACATTGCGTCCA	50.0	45.0	58.0	57.3	386-401 bp	1.0	79.6
trnS-CGA-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	ATGGGAATACATTGCGTCCA	55.0	45.0	58.1	57.3	389-404 bp	1.0	79.4
trnS-CGA-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	TGGGAATACATTGCGTCCAA	55.0	45.0	57.7	57.5	389-404 bp	1.0	79.0