Hotspot

rps16-trnQ-UUG

LSC · 5752-7270 · 1519 bp

Back to Omphalogramma

Pi 0.0149
MarkerSeek score 60.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1519
variable_sites
59
pi
0.0149
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1593-1630 bp 1.0 82.7
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1595-1632 bp 1.0 81.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1586-1623 bp 1.0 81.7
rps16-trnQ-UUG_p4 GTCGAGTCAAGAGCACCTTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1657-1694 bp 1.0 81.4
rps16-trnQ-UUG_p5 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1596-1633 bp 1.0 81.0