Hotspot

rps16

LSC · 5163-6283 · 1121 bp

Back to Omalotheca

Pi 0

MarkerSeek score 58.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1121
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAGCAAGAAAAGAAGG	ACTGGTTCCAAATTCGAGGA	39.1	45.0	56.9	56.8	1248-1250 bp	1.0	40.1
rps16_p2	ACGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGA	37.5	45.0	58.2	56.8	1248-1250 bp	1.0	40.1
rps16_p3	CGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGA	39.1	45.0	56.6	56.8	1247-1249 bp	1.0	40.1
rps16_p4	ACGAATAGAGCAAGAAAAGAAGG	ACTGGTTCCAAATTCGAGGAA	39.1	42.9	56.9	57.5	1248-1250 bp	1.0	40.1
rps16_p5	ACGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGAA	37.5	42.9	58.2	57.5	1248-1250 bp	1.0	40.1