Hotspot

psbH

LSC · 74510-74731 · 222 bp

Back to Omalotheca

Pi 0

MarkerSeek score 19.80

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	AACCGCCGAGAATTCAAACT	CCTTACGGGCCTCTTGAATC	45.0	55.0	58.1	58.0	1241 bp	1.0	78.8
psbH_p2	AACCGCCGAGAATTCAAACT	CTTACGGGCCTCTTGAATCC	45.0	55.0	58.1	58.0	1240 bp	1.0	78.8
psbH_p3	TGTTGAGAAGGTTGCCCAAA	CCTTACGGGCCTCTTGAATC	45.0	55.0	57.8	58.0	1095 bp	1.0	78.5
psbH_p4	TGTTGAGAAGGTTGCCCAAA	CTTACGGGCCTCTTGAATCC	45.0	55.0	57.8	58.0	1094 bp	1.0	78.5
psbH_p5	TTGTTGAGAAGGTTGCCCAA	CTTACGGGCCTCTTGAATCC	45.0	55.0	57.8	58.0	1095 bp	1.0	78.5