Hotspot

psbA

LSC · 471-1532 · 1062 bp

Back to Omalotheca

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GCAATAGCTTCCCTCTTGAT	TGGTTGACACGCGCATATAA	45.0	45.0	55.2	58.0	1262 bp	1.0	57.4
psbA_p2	GCAATAGCTTCCCTCTTGAT	TTGGTTGACACGCGCATATA	45.0	45.0	55.2	58.0	1263 bp	1.0	57.4
psbA_p3	GCAATAGCTTCCCTCTTGAT	AAATTCGTGTGCTTGGGAGT	45.0	45.0	55.2	58.0	1197 bp	1.0	57.4
psbA_p4	GCAATAGCTTCCCTCTTGAT	TGCTTGGGAGTCCCTGATAA	45.0	50.0	55.2	58.0	1188 bp	1.0	57.2
psbA_p5	GCAATAGCTTCCCTCTTGAT	AATTCGTGTGCTTGGGAGTC	45.0	50.0	55.2	58.5	1196 bp	1.0	53.7