Hotspot

petD

LSC · 76456-77644 · 1189 bp

Back to Omalotheca

Pi 0.000841

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1189
variable_sites: 1
pi: 0.000841
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GAACTCCGCTTTGATTTGGC	AAGCGACCGTTCCCTAGATA	50.0	50.0	58.0	57.9	1306 bp	1.0	79.4
petD_p2	GAACTCCGCTTTGATTTGGC	ATTCACTTTGAAGCGACCGT	50.0	45.0	58.0	58.1	1316 bp	1.0	79.0
petD_p3	CCCTTGAACTCCGCTTTGAT	AAGCGACCGTTCCCTAGATA	50.0	50.0	58.2	57.9	1311 bp	1.0	78.2
petD_p4	CTCCGCTTTGATTTGGCATC	AAGCGACCGTTCCCTAGATA	50.0	50.0	57.8	57.9	1303 bp	1.0	78.0
petD_p5	CCCTTGAACTCCGCTTTGAT	ATTCACTTTGAAGCGACCGT	50.0	45.0	58.2	58.1	1321 bp	1.0	77.7