Hotspot

ndhD

SSC · 119703-121325 · 1623 bp

Back to Omalotheca

Pi 0.00185

MarkerSeek score 59.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1623
variable_sites: 3
pi: 0.00185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACAGAGGATTGTGTCGGTTG	TCATTAGCCAACCGACTCTT	50.0	45.0	57.8	56.6	1789 bp	1.0	67.2
ndhD_p2	ACAGAGGATTGTGTCGGTTG	TTCATTAGCCAACCGACTCT	50.0	45.0	57.8	56.6	1790 bp	1.0	67.2
ndhD_p3	CAGAGGATTGTGTCGGTTGT	TCATTAGCCAACCGACTCTT	50.0	45.0	57.8	56.6	1788 bp	1.0	67.2
ndhD_p4	CAGAGGATTGTGTCGGTTGT	TTCATTAGCCAACCGACTCT	50.0	45.0	57.8	56.6	1789 bp	1.0	67.2
ndhD_p5	TTATGGCATGAAACAACGCG	TCATTAGCCAACCGACTCTT	45.0	45.0	57.7	56.6	1717 bp	1.0	66.6