Hotspot

atpB-rbcL

LSC · 54536-55277 · 742 bp

Back to Omalotheca

Pi 0.00135

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 1
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGCCTGGCGGAAAAGATA	CACCTGCTTTGAATCCAACAC	45.0	47.6	57.8	58.0	899 bp	1.0	70.3
atpB-rbcL_p2	TTTGCCTGGCGGAAAAGATA	ACCTGCTTTGAATCCAACACT	45.0	42.9	57.8	58.1	898 bp	1.0	70.0
atpB-rbcL_p3	TTTGCCTGGCGGAAAAGATA	TGCTTTGAATCCAACACTTGC	45.0	42.9	57.8	57.9	895 bp	1.0	69.7
atpB-rbcL_p4	GCCTGGCGGAAAAGATACAT	CACCTGCTTTGAATCCAACAC	50.0	47.6	58.3	58.0	896 bp	1.0	69.4
atpB-rbcL_p5	GCCTGGCGGAAAAGATACAT	ACCTGCTTTGAATCCAACACT	50.0	42.9	58.3	58.1	895 bp	1.0	69.1