Hotspot

matK-trnK-UUU

LSC · 3593-4325 · 733 bp

Back to Omalotheca

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TGTTGTTGAGATCTATCTAGCCC	AACGGTAGAGTACTCGGCTT	43.5	50.0	57.5	58.2	804 bp	1.0	50.4
matK-trnK-UUU_p2	TGTTGTTGAGATCTATCTAGCCC	ACGGTAGAGTACTCGGCTTT	43.5	50.0	57.5	58.2	803 bp	1.0	50.4
matK-trnK-UUU_p3	TCTAGCCCTAAATAGCTTTGGA	AACGGTAGAGTACTCGGCTT	40.9	50.0	56.3	58.2	789 bp	1.0	49.3
matK-trnK-UUU_p4	TCTAGCCCTAAATAGCTTTGGA	ACGGTAGAGTACTCGGCTTT	40.9	50.0	56.3	58.2	788 bp	1.0	49.3
matK-trnK-UUU_p5	AGCCCTAAATAGCTTTGGAA	ACGGTAGAGTACTCGGCTTT	40.0	50.0	54.3	58.2	785 bp	1.0	48.9