Hotspot

rps16

LSC · 4530-5616 · 1087 bp

Back to Oligostachyum

Pi 0.000736

MarkerSeek score 47.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1087
variable_sites: 4
pi: 0.000736
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGA	45.0	40.9	57.2	57.1	1241-1248 bp	1.0	51.7
rps16_p2	TGGCACGACATAAATCCAGT	TCAATTCCCTATACCACTTTGCA	45.0	39.1	57.2	57.8	1246-1253 bp	1.0	49.0
rps16_p3	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGAA	45.0	39.1	57.2	57.8	1241-1248 bp	1.0	49.0
rps16_p4	TGGCACGACATAAATCCAGT	TTCCCTATACCACTTTGCAATGA	45.0	39.1	57.2	57.8	1242-1249 bp	1.0	49.0
rps16_p5	TGGCACGACATAAATCCAGT	CCCTATACCACTTTGCAATGA	45.0	42.9	57.2	55.6	1240-1247 bp	1.0	47.6