Hotspot

psbD

LSC · 9459-10520 · 1062 bp

Back to Oligostachyum

Pi 0.000753

MarkerSeek score 46.40

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 4
pi: 0.000753
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AGTGATATGAGGTGCTCGGA	GTCACGACCAGCTAAAACGA	50.0	50.0	57.9	58.2	1142 bp	1.0	77.9
psbD_p2	TGCTCGGAAATGGTTGAAGT	GTCACGACCAGCTAAAACGA	45.0	50.0	57.7	58.2	1130 bp	1.0	76.3
psbD_p3	AGTGATATGAGGTGCTCGGA	TCACGACCAGCTAAAACGAA	50.0	45.0	57.9	57.5	1141 bp	1.0	75.6
psbD_p4	TGCTCGGAAATGGTTGAAGT	TCACGACCAGCTAAAACGAA	45.0	45.0	57.7	57.5	1129 bp	1.0	74.0
psbD_p5	GTGCTCGGAAATGGTTGAAG	GTCACGACCAGCTAAAACGA	50.0	50.0	57.4	58.2	1131 bp	1.0	73.5