Hotspot

petD

LSC · 75512-76743 · 1232 bp

Back to Oligostachyum

Pi 0.000812

MarkerSeek score 44.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1232
variable_sites: 5
pi: 0.000812
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGATACTTTTCTTCAACTCCGA	CACGGTACTTCACGGTTGAA	39.1	50.0	57.5	58.1	1350-1355 bp	1.0	51.3
petD_p2	ACTTTTCTTCAACTCCGAAGT	CACGGTACTTCACGGTTGAA	38.1	50.0	55.4	58.1	1345-1350 bp	1.0	50.2
petD_p3	TGCTACAAACATGGGTTATTGT	CACGGTACTTCACGGTTGAA	36.4	50.0	56.2	58.1	1390-1395 bp	1.0	49.1
petD_p4	TGGATACTTTTCTTCAACTCCGA	CCTATGCACGGTACTTCACG	39.1	55.0	57.5	58.4	1356-1361 bp	1.0	48.9
petD_p5	TGGATACTTTTCTTCAACTCCGA	CTATGCACGGTACTTCACGG	39.1	55.0	57.5	58.4	1355-1360 bp	1.0	48.9