Hotspot

trnN-GUU-rps15

IRb · 102933-104473 · 1541 bp

Back to Oligostachyum

Pi 0.000649

MarkerSeek score 46.20

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1541
variable_sites: 5
pi: 0.000649
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	ACGACCAGTCAGTTAACAGC	CAGAACCCCTGTTTTCTTCT	50.0	45.0	57.8	55.2	1663 bp	1.0	56.4
trnN-GUU-rps15_p2	AGGATTCGAACCTACGACCA	CAGAACCCCTGTTTTCTTCT	50.0	45.0	58.2	55.2	1676 bp	1.0	56.3
trnN-GUU-rps15_p3	GGATTCGAACCTACGACCAG	CAGAACCCCTGTTTTCTTCT	55.0	45.0	57.8	55.2	1675 bp	1.0	55.9
trnN-GUU-rps15_p4	ACGACCAGTCAGTTAACAGC	ACTTGAAATTCAACAGAACCCC	50.0	40.9	57.8	57.1	1676 bp	1.0	55.5
trnN-GUU-rps15_p5	AGGATTCGAACCTACGACCA	ACTTGAAATTCAACAGAACCCC	50.0	40.9	58.2	57.1	1689 bp	1.0	55.5