Hotspot

psaJ-rpl33

LSC · 67342-67787 · 446 bp

Back to Oligostachyum

Pi 0.00309

MarkerSeek score 51.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 6
pi: 0.00309
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTC	45.0	55.0	57.9	58.1	604-613 bp	1.0	80.1
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	GGCACCTTTTCGAACACAAC	45.0	50.0	57.9	58.2	581-590 bp	1.0	80.0
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	CGATTCCTCATTGGCACCTT	45.0	50.0	57.9	58.2	593-602 bp	1.0	79.3
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CTAACACCTTTCCCCTTGGC	45.0	55.0	57.9	58.5	535-544 bp	1.0	77.8
psaJ-rpl33_p5	TTTGGGGCTTTAGCAGGTTT	CCTCATTGGCACCTTTTCGA	45.0	50.0	57.9	58.5	588-597 bp	1.0	77.5