Hotspot

psbA

LSC · 437-1498 · 1062 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCAATAACGCCCTCTTGTT	TTGAAAATTCGTGCGCTTGG	45.0	45.0	57.8	58.2	1202 bp	1.0	76.5
psbA_p2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	45.0	45.0	57.8	58.2	1208 bp	1.0	76.4
psbA_p3	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	50.0	45.0	58.2	58.2	1207 bp	1.0	76.1
psbA_p4	GAGCAATAACGCCCTCTTGT	TTGAAAATTCGTGCGCTTGG	50.0	45.0	58.3	58.2	1203 bp	1.0	76.0
psbA_p5	AGCAATAACGCCCTCTTGTT	CCATTTGAAAATTCGTGCGC	45.0	45.0	57.8	57.2	1206 bp	1.0	72.3