Hotspot

trnE-UUC-rpoB

LSC · 11690-12496 · 807 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GAGAGAAGTCGTAACCGCTG	CATTGCGTCTATCCCGGATT	55.0	50.0	58.1	57.8	893 bp	1.0	77.8
trnE-UUC-rpoB_p2	AACCCTCGTTATCTCCGTGA	CATTGCGTCTATCCCGGATT	50.0	50.0	58.2	57.8	915 bp	1.0	77.4
trnE-UUC-rpoB_p3	ACCCTCGTTATCTCCGTGAA	CATTGCGTCTATCCCGGATT	50.0	50.0	58.2	57.8	914 bp	1.0	77.4
trnE-UUC-rpoB_p4	TGAACCCTCGTTATCTCCGT	CATTGCGTCTATCCCGGATT	50.0	50.0	58.2	57.8	917 bp	1.0	77.4
trnE-UUC-rpoB_p5	AGAGAGAAGTCGTAACCGCT	CATTGCGTCTATCCCGGATT	50.0	50.0	58.2	57.8	894 bp	1.0	77.2