Hotspot

psbM-trnD-GUC

LSC · 10576-11241 · 666 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.6	58.0	805 bp	1.0	52.4
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	40.0	50.0	54.5	58.0	799 bp	1.0	51.9
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.4	58.0	802 bp	1.0	51.0
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	783 bp	1.0	50.4
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCAG	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.3	58.0	806 bp	1.0	50.3