Hotspot

matK-trnK-UUU

LSC · 3550-4283 · 734 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.5	58.2	866 bp	1.0	58.7
matK-trnK-UUU_p2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.5	58.2	865 bp	1.0	58.7
matK-trnK-UUU_p3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	40.9	50.0	57.5	58.4	881 bp	1.0	56.9
matK-trnK-UUU_p4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	40.9	50.0	57.5	57.5	879 bp	1.0	56.4
matK-trnK-UUU_p5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	40.0	50.0	55.0	58.2	866 bp	1.0	54.7