primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TGGAAGTGATGCATGAACGT	20	20	55.0	45.0	58.1	57.8	0.0	2.5	0.312398	78	436	460-460	1.000000	77.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TGGAAGTGATGCATGAACGT	20	20	50.0	45.0	57.9	57.8	0.0	2.5	0.316760	78	436	474-474	1.000000	77.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TGGAAGTGATGCATGAACGT	20	20	50.0	45.0	57.9	57.8	0.0	2.5	0.318267	78	436	473-473	1.000000	77.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TGGAAGTGATGCATGAACGT	20	20	50.0	45.0	57.9	57.8	0.0	2.5	0.318267	78	436	475-475	1.000000	77.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	436	477-477	1.000000	76.3
psbA_p1	psbA	1	AGCAATAACGCCCTCTTGTT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.435522	437	1498	1202-1202	1.000000	76.5
psbA_p2	psbA	2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.451091	437	1498	1208-1208	1.000000	76.4
psbA_p3	psbA	3	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.2	58.2	0.0	9.7	0.483712	437	1498	1207-1207	1.000000	76.1
psbA_p4	psbA	4	GAGCAATAACGCCCTCTTGT	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.3	58.2	0.0	9.7	0.496082	437	1498	1203-1203	1.000000	76.0
psbA_p5	psbA	5	AGCAATAACGCCCTCTTGTT	CCATTTGAAAATTCGTGCGC	20	20	45.0	45.0	57.8	57.2	0.0	0.0	0.967938	437	1498	1206-1206	1.000000	72.3
matK_p1	matK	1	GTGATCAAATTTTTATTTACAACG	CCCCCAAATCCTCTACCTTTC	24	21	25.0	52.4	52.1	57.7	0.0	0.0	11.201679	2029	3549	1596-1596	1.000000	40.0
matK_p2	matK	2	GTGATCAAATTTTTATTTACAACG	CCCCCAAATCCTCTACCTTT	24	20	25.0	50.0	52.1	56.5	0.0	0.0	11.428586	2029	3549	1596-1596	1.000000	40.0
matK_p3	matK	3	GTGATCAAATTTTTATTTACAACG	CCCCAAATCCTCTACCTTTCA	24	21	25.0	47.6	52.1	57.0	0.0	0.0	11.923988	2029	3549	1595-1595	1.000000	40.0
matK_p4	matK	4	GTGATCAAATTTTTATTTACAACG	CCTTGTTTTGACTGTATCGCA	24	21	25.0	42.9	52.1	56.8	0.0	0.0	12.070481	2029	3549	1636-1636	1.000000	40.0
matK_p5	matK	5	GTGATCAAATTTTTATTTACAACG	ACCTTGTTTTGACTGTATCGC	24	21	25.0	42.9	52.1	56.8	0.0	0.0	12.072514	2029	3549	1637-1637	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3550	4283	866-866	1.000000	58.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3550	4283	865-865	1.000000	58.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3550	4283	881-881	1.000000	56.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3550	4283	879-879	1.000000	56.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3550	4283	866-866	1.000000	54.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4321	5099	976-976	1.000000	76.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4321	5099	975-975	1.000000	76.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTCGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.9	58.3	0.0	7.6	0.443468	4321	5099	1000-1000	1.000000	76.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTCGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.9	58.3	0.0	7.6	0.449310	4321	5099	999-999	1.000000	76.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4321	5099	914-914	1.000000	76.1
rps16_p1	rps16	1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.9	57.7	0.0	0.0	4.403459	5100	6208	1295-1295	1.000000	44.8
rps16_p2	rps16	2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	58.2	57.7	0.0	0.0	4.486645	5100	6208	1295-1295	1.000000	44.1
rps16_p3	rps16	3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.6	57.7	0.0	0.0	4.654102	5100	6208	1294-1294	1.000000	42.8
rps16_p4	rps16	4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	23	20	39.1	55.0	56.9	57.4	0.0	0.0	4.755119	5100	6208	1296-1296	1.000000	42.0
rps16_p5	rps16	5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	58.2	57.4	0.0	0.0	4.838305	5100	6208	1296-1296	1.000000	41.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6209	7147	1012-1012	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6209	7147	1027-1027	1.000000	76.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6209	7147	1026-1026	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6209	7147	1083-1083	1.000000	75.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6209	7147	1016-1016	1.000000	73.5
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	GTTTGTTTATGAGGCGGCAC	20	20	50.0	50.0	58.2	58.0	0.0	13.1	0.174160	8481	9251	942-942	1.000000	78.6
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8481	9251	922-922	1.000000	78.1
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8481	9251	904-904	1.000000	75.9
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	CTTTCGTCCACTCAGCCATC	GTTTGTTTATGAGGCGGCAC	20	20	55.0	50.0	58.6	58.0	0.0	13.1	0.659068	8481	9251	878-878	1.000000	74.7
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	AACGGAAAGAGAGGGATTCG	GTTTGTTTATGAGGCGGCAC	20	20	50.0	50.0	57.3	58.0	0.0	13.1	0.697607	8481	9251	943-943	1.000000	74.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TCTGATTCCCCTGGGATTGT	23	20	39.1	50.0	57.6	58.0	0.0	16.2	3.453139	10576	11241	805-805	1.000000	52.4
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	20	20	40.0	50.0	54.5	58.0	0.0	16.2	3.508990	10576	11241	799-799	1.000000	51.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	23	20	39.1	50.0	57.4	58.0	0.0	16.2	3.627737	10576	11241	802-802	1.000000	51.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10576	11241	783-783	1.000000	50.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAAAATGAACAGTGCAG	TCTGATTCCCCTGGGATTGT	23	20	39.1	50.0	57.3	58.0	0.0	16.2	3.710230	10576	11241	806-806	1.000000	50.3
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GAGAGAAGTCGTAACCGCTG	CATTGCGTCTATCCCGGATT	20	20	55.0	50.0	58.1	57.8	0.0	0.7	0.272191	11690	12496	893-893	1.000000	77.8
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	AACCCTCGTTATCTCCGTGA	CATTGCGTCTATCCCGGATT	20	20	50.0	50.0	58.2	57.8	0.0	0.7	0.326464	11690	12496	915-915	1.000000	77.4
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	ACCCTCGTTATCTCCGTGAA	CATTGCGTCTATCCCGGATT	20	20	50.0	50.0	58.2	57.8	0.0	0.7	0.326464	11690	12496	914-914	1.000000	77.4
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	TGAACCCTCGTTATCTCCGT	CATTGCGTCTATCCCGGATT	20	20	50.0	50.0	58.2	57.8	0.0	0.7	0.326464	11690	12496	917-917	1.000000	77.4
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	AGAGAGAAGTCGTAACCGCT	CATTGCGTCTATCCCGGATT	20	20	50.0	50.0	58.2	57.8	0.0	0.7	0.346035	11690	12496	894-894	1.000000	77.2
rps2_p1	rps2	1	TTTTTCAAAAGGGGGAGGCA	TCTATGAAGGTTCCGAAATGA	20	21	45.0	38.1	57.8	54.4	0.0	0.0	4.813180	23039	23749	789-789	1.000000	41.5
rps2_p2	rps2	2	TTTTTCAAAAGGGGGAGGCA	TCGATAAATCTATGAAGGTTCCGA	20	24	45.0	37.5	57.8	57.4	0.0	0.0	4.826764	23039	23749	797-797	1.000000	41.4
rps2_p3	rps2	3	TTTTCAAAAGGGGGAGGCAG	TCTATGAAGGTTCCGAAATGA	20	21	50.0	38.1	58.3	54.4	0.0	0.0	4.914879	23039	23749	788-788	1.000000	40.7
rps2_p4	rps2	4	TTTTCAAAAGGGGGAGGCAG	TCGATAAATCTATGAAGGTTCCGA	20	24	50.0	37.5	58.3	57.4	0.0	0.0	4.928463	23039	23749	796-796	1.000000	40.6
rps2_p5	rps2	5	TTTTTCAAAAGGGGGAGGCA	TCTATGAAGGTTCCGAAATGAA	20	22	45.0	36.4	57.8	55.2	0.0	0.0	4.995709	23039	23749	789-789	1.000000	40.0