Hotspot

rps15-trnN(GUU)

SSC · 124558-130886 · 6329 bp

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Pi 0.00886
MarkerSeek score 48.50
Primer available yes
Label rps15-trnN(GUU)

Feature Metadata

label_name
rps15-trnN(GUU)
feature_type
igs
region
SSC
length_bp
6329
variable_sites
254
pi
0.00886
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN(GUU)_p1 CTGACAGCGTTTTCAAATCGA AGCCCAGTTTATGAAGATTCT 42.9 38.1 57.5 54.6 200-212 bp 1.0 48.9
rps15-trnN(GUU)_p2 TCTGACAGCGTTTTCAAATCG AGCCCAGTTTATGAAGATTCT 42.9 38.1 57.5 54.6 201-213 bp 1.0 48.9
rps15-trnN(GUU)_p3 CTGACAGCGTTTTCAAATCGA AGCCCAGTTTATGAAGATTCTT 42.9 36.4 57.5 55.4 200-212 bp 1.0 48.9
rps15-trnN(GUU)_p4 TCTGACAGCGTTTTCAAATCG AGCCCAGTTTATGAAGATTCTT 42.9 36.4 57.5 55.4 201-213 bp 1.0 48.9
rps15-trnN(GUU)_p5 TGACAGCGTTTTCAAATCGA AGCCCAGTTTATGAAGATTCT 40.0 38.1 56.3 54.6 199-211 bp 1.0 48.6