Hotspot

psbE-petL

LSC · 66708-67873 · 1166 bp

Pi 0.00795

MarkerSeek score 59.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 47
pi: 0.00795
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1352-1375 bp	1.0	76.5
psbE-petL_p2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1365-1388 bp	1.0	76.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1275-1298 bp	1.0	75.9
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1361-1384 bp	1.0	75.5
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1360-1383 bp	1.0	75.4