Hotspot

ndhC-trnM(CAU)

LSC · 51942-53888 · 1947 bp

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Pi 0.00699
MarkerSeek score 62.60
Primer available yes
Label ndhC-trnM(CAU)

Feature Metadata

label_name
ndhC-trnM(CAU)
feature_type
igs
region
LSC
length_bp
1947
variable_sites
63
pi
0.00699
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM(CAU)_p1 GGGGCTAAAATCCCGGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 2068-2092 bp 1.0 80.1
ndhC-trnM(CAU)_p2 GGGGCTAAAATCCCGGAAAT AGGTACCATTGACTCCGGTA 50.0 50.0 57.9 57.5 2129-2153 bp 1.0 79.0
ndhC-trnM(CAU)_p3 GGGGCTAAAATCCCGGAAAT TAGGTACCATTGACTCCGGT 50.0 50.0 57.9 57.5 2130-2154 bp 1.0 79.0
ndhC-trnM(CAU)_p4 GGGGCTAAAATCCCGGAAAT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.9 57.3 2067-2091 bp 1.0 78.1
ndhC-trnM(CAU)_p5 TTCTCCGGCCCTTTCCTAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 58.4 58.4 2089-2113 bp 1.0 77.4