Hotspot

psbM-trnD(GUC)

LSC · 30884-31530 · 647 bp

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Pi 0.0151
MarkerSeek score 65.30
Primer available yes
Label psbM-trnD(GUC)

Feature Metadata

label_name
psbM-trnD(GUC)
feature_type
igs
region
LSC
length_bp
647
variable_sites
36
pi
0.0151
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD(GUC)_p1 AGAATGAATAGTGCAGTCGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.9 58.5 636-759 bp 1.0 67.6
psbM-trnD(GUC)_p2 AGAATGAATAGTGCAGTCGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.9 57.0 637-760 bp 1.0 63.4
psbM-trnD(GUC)_p3 AGTAGAAAAGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 42.9 50.0 56.4 58.5 657-780 bp 1.0 63.1
psbM-trnD(GUC)_p4 AGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 45.0 50.0 55.4 58.5 649-772 bp 1.0 62.9
psbM-trnD(GUC)_p5 AGAATGAATAGTGCAGTCGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.9 57.8 635-758 bp 1.0 61.6