Hotspot

rps2-rpoC2

LSC · 17034-17241 · 208 bp

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Pi 0.0188
MarkerSeek score 61.00
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
208
variable_sites
17
pi
0.0188
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 TGCCTCCATCATCTCTTCCA TTCGAGGGGGAAATGAGAGA 50.0 50.0 58.1 57.8 369-381 bp 1.0 82.7
rps2-rpoC2_p2 AACTCCTGCCTCCATCATCT TTCGAGGGGGAAATGAGAGA 50.0 50.0 58.1 57.8 375-387 bp 1.0 82.6
rps2-rpoC2_p3 TCCTGCCTCCATCATCTCTT TTCGAGGGGGAAATGAGAGA 50.0 50.0 57.8 57.8 372-384 bp 1.0 82.0
rps2-rpoC2_p4 GAACTCCTGCCTCCATCATC TTCGAGGGGGAAATGAGAGA 55.0 50.0 57.7 57.8 376-388 bp 1.0 81.5
rps2-rpoC2_p5 CTCCTGCCTCCATCATCTCT TTCGAGGGGGAAATGAGAGA 55.0 50.0 58.3 57.8 373-385 bp 1.0 81.3