Hotspot

psbI-trnS(GCU)

LSC · 8800-8925 · 126 bp

Back to Olea

Pi 0.0397
MarkerSeek score 68.30
Primer available yes
Label psbI-trnS(GCU)

Feature Metadata

label_name
psbI-trnS(GCU)
feature_type
igs
region
LSC
length_bp
126
variable_sites
30
pi
0.0397
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS(GCU)_p1 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 211-223 bp 1.0 84.5
psbI-trnS(GCU)_p2 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 212-224 bp 1.0 84.5
psbI-trnS(GCU)_p3 CGTAATCCTGGACGTGAAGA ATTGGGAGAGATGGCTGAGT 50.0 50.0 57.1 58.1 230-242 bp 1.0 83.7
psbI-trnS(GCU)_p4 CGTAATCCTGGACGTGAAGAA TGGACTAAAGCGTCGGATTG 47.6 50.0 57.8 58.0 211-223 bp 1.0 82.2
psbI-trnS(GCU)_p5 CGTAATCCTGGACGTGAAGAA GTGGACTAAAGCGTCGGATT 47.6 50.0 57.8 58.0 212-224 bp 1.0 82.1