Hotspot

trnK-rps16

LSC · 4407-5290 · 884 bp

Back to Olea

Pi 0.0141
MarkerSeek score 55.80
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
884
variable_sites
49
pi
0.0141
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 CGAATTCCTCGCTTCATCCA AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1602-1713 bp 1.0 86.5
trnK-rps16_p2 CGAATTCCTCGCTTCATCCA TCTATCCCAATGAGCCGTCT 50.0 50.0 58.1 57.9 1133-1229 bp 1.0 86.2
trnK-rps16_p3 CGAATTCCTCGCTTCATCCA GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1189-1285 bp 1.0 86.1
trnK-rps16_p4 TTCCGTTCCACGAATTCGAA AAAAGGGTATGTTGCTGCCA 45.0 45.0 57.8 58.0 1751-1862 bp 1.0 85.6
trnK-rps16_p5 CGAATTCCTCGCTTCATCCA CCCAGAACAAGGAAAGACCC 50.0 55.0 58.1 58.1 1587-1589 bp 0.086957 42.3