Hotspot

ndhI

SSC · 114036-114578 · 543 bp

Back to Oldeania

Pi 0.00276

MarkerSeek score 36.30

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 3
pi: 0.00276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AAGTTGTGCGACAGCTACAA	ACTTAGGCGGGTGGAATTTC	45.0	50.0	58.1	57.9	1340 bp	1.0	79.4
ndhI_p2	AGTTGTGCGACAGCTACAAA	ACTTAGGCGGGTGGAATTTC	45.0	50.0	58.1	57.9	1339 bp	1.0	79.4
ndhI_p3	TTGTGCGACAGCTACAAAGT	ACTTAGGCGGGTGGAATTTC	45.0	50.0	58.1	57.9	1337 bp	1.0	79.4
ndhI_p4	AGTTGTGCGACAGCTACAAA	TCTACTTAGGCGGGTGGAAT	45.0	50.0	58.1	57.8	1342 bp	1.0	79.0
ndhI_p5	TTGTGCGACAGCTACAAAGT	TCTACTTAGGCGGGTGGAAT	45.0	50.0	58.1	57.8	1340 bp	1.0	79.0