Hotspot

ndhA

SSC · 114672-116786 · 2115 bp

Back to Oldeania

Pi 0.00418

MarkerSeek score 60.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 17
pi: 0.00418
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2252 bp	1.0	71.0
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2250 bp	1.0	70.4
ndhA_p3	TGAACCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	57.8	57.9	2255 bp	1.0	69.9
ndhA_p4	TGAACCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	57.8	57.8	2253 bp	1.0	69.4
ndhA_p5	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2251 bp	1.0	65.3