Hotspot

ndhF-rpl32

SSC · 107351-108229 · 879 bp

Back to Oldeania

Pi 0.00342

MarkerSeek score 61.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 879
variable_sites: 6
pi: 0.00342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	990-1001 bp	1.0	41.9
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	962-973 bp	1.0	41.6
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	963-974 bp	1.0	41.6
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	964-975 bp	1.0	41.6
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	965-976 bp	1.0	41.6