Hotspot

rpl22-rps19

IRb · 83181-83244 · 64 bp

Back to Oldeania

Pi 0.0234

MarkerSeek score 59.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 64
variable_sites: 3
pi: 0.0234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGAGTTTCCTTAGCCCACTC	CAAATTGGGGGAATTCGTGC	55.0	50.0	58.0	58.0	213-214 bp	1.0	81.0
rpl22-rps19_p2	CGAGTTTCCTTAGCCCACTC	GCAAATTGGGGGAATTCGTG	55.0	50.0	58.0	58.0	214-215 bp	1.0	81.0
rpl22-rps19_p3	TCCTTGCGAGTTTCCTTAGC	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	219-220 bp	1.0	80.3
rpl22-rps19_p4	TCCTTGCGAGTTTCCTTAGC	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	220-221 bp	1.0	80.3
rpl22-rps19_p5	CGAGTTTCCTTAGCCCACTC	ATGGTAGGTCGCAAATTGGG	55.0	50.0	58.0	58.2	224-225 bp	1.0	79.1