Hotspot

ndhC-trnV-UAC

LSC · 52427-53178 · 752 bp

Back to Oldeania

Pi 0.00842
MarkerSeek score 51.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
752
variable_sites
11
pi
0.00842
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 797-801 bp 1.0 49.4
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 798-802 bp 1.0 47.6
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 796-800 bp 1.0 45.4
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 798-802 bp 1.0 45.4
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 797-801 bp 1.0 45.4