Hotspot

ycf3-trnS-GGA

LSC · 46359-46949 · 591 bp

Back to Oldeania

Pi 0.00423

MarkerSeek score 58.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 5
pi: 0.00423
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	778 bp	1.0	79.1
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	728 bp	1.0	78.8
ycf3-trnS-GGA_p3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	730 bp	1.0	76.0
ycf3-trnS-GGA_p4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	50.0	50.0	58.0	58.5	731 bp	1.0	76.0
ycf3-trnS-GGA_p5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	50.0	50.0	58.0	58.6	727 bp	1.0	75.9