Hotspot

atpF

LSC · 52721-54038 · 1318 bp

Back to Oenothera

Pi 0.000624

MarkerSeek score 45.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1318
variable_sites: 3
pi: 0.000624
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCAAAAAGAAATAGGAATAGCAA	TTCGTCGGCTCTAATGGTTG	26.1	50.0	52.3	58.0	1488-1501 bp	1.0	40.9
atpF_p2	TTCAAAAAGAAATAGGAATAGCA	TTCGTCGGCTCTAATGGTTG	26.1	50.0	52.3	58.0	1489-1502 bp	1.0	40.9
atpF_p3	TCAAAAAGAAATAGGAATAGCAA	GGCTCTAATGGTTGCCATGA	26.1	50.0	52.3	57.9	1482-1495 bp	1.0	40.9
atpF_p4	TTCAAAAAGAAATAGGAATAGCA	GGCTCTAATGGTTGCCATGA	26.1	50.0	52.3	57.9	1483-1496 bp	1.0	40.9
atpF_p5	AGAAAAAGTGTTTAAGTGATACA	TTCGTCGGCTCTAATGGTTG	26.1	50.0	52.3	58.0	1460-1473 bp	1.0	40.9