Hotspot

ycf2-trnICAU

IRa · 162248-162831 · 584 bp

Back to Oenothera

Pi 0.00815

MarkerSeek score 41.70

Primer available yes

Label ycf2-trnICAU

Feature Metadata

label_name: ycf2-trnICAU
feature_type: igs
region: IRa
length_bp: 584
variable_sites: 10
pi: 0.00815
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnICAU_p1	AACGCAGGACACTCCAAATT	GTTGGGCGCTTTAACCATTC	45.0	50.0	58.0	58.0	678-826 bp	1.0	86.1
ycf2-trnICAU_p2	GCAGGAGCGAAACAATCAAC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	879-1027 bp	1.0	85.5
ycf2-trnICAU_p3	AGGAAGATCCAGAAAGGCCT	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1197-1345 bp	1.0	85.2
ycf2-trnICAU_p4	AACGCAGGACACTCCAAATT	CACTTGACTGTCTACGGCTC	45.0	55.0	58.0	58.0	1274-1422 bp	1.0	85.1
ycf2-trnICAU_p5	AGGAAGATCCAGAAAGGCCT	CACTTGACTGTCTACGGCTC	50.0	55.0	58.0	58.0	1793-1941 bp	1.0	84.7