Hotspot

ndhF-rpl32

SSC · 117403-118282 · 880 bp

Back to Oenothera

Pi 0.0072

MarkerSeek score 53.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 880
variable_sites: 16
pi: 0.0072
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTGGTAGTGGAATGAAAGGT	TTTGATATAGAAGTGCGTTTTT	42.9	27.3	56.5	52.4	937-1000 bp	1.0	45.3
ndhF-rpl32_p2	ACTGGTAGTGGAATGAAAGGT	TTTTGATATAGAAGTGCGTTTT	42.9	27.3	56.5	52.4	938-1001 bp	1.0	45.3
ndhF-rpl32_p3	ACTGGTAGTGGAATGAAAGGT	TTTTTGATATAGAAGTGCGTTT	42.9	27.3	56.5	52.4	939-1002 bp	1.0	45.3
ndhF-rpl32_p4	ACTGGTAGTGGAATGAAAGGT	TCCAAATAGTTTTACGAATACG	42.9	31.8	56.5	52.4	963-1026 bp	1.0	45.3
ndhF-rpl32_p5	AGGAACTGGTAGTGGAATGA	TTTGATATAGAAGTGCGTTTTT	45.0	27.3	55.5	52.4	941-1004 bp	1.0	45.3