Hotspot

rrn16-trnIGAU

IRb · 107472-108371 · 900 bp

Back to Oenothera

Pi 0.0275

MarkerSeek score 62.20

Primer available yes

Label rrn16-trnIGAU

Feature Metadata

label_name: rrn16-trnIGAU
feature_type: igs
region: IRb
length_bp: 900
variable_sites: 46
pi: 0.0275
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnIGAU_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	543-1045 bp	1.0	84.4
rrn16-trnIGAU_p2	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	562-1064 bp	1.0	84.3
rrn16-trnIGAU_p3	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	511-1013 bp	1.0	84.3
rrn16-trnIGAU_p4	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	50.0	55.0	57.6	57.8	552-1054 bp	1.0	83.8
rrn16-trnIGAU_p5	AAGTCGTAACAAGGTAGCCG	CGCTGATGAGCACATTGAAC	50.0	50.0	57.6	57.8	571-1073 bp	1.0	83.7