Hotspot

trnICAU-ycf2

IRb · 90744-91327 · 584 bp

Back to Oenothera

Pi 0.00821

MarkerSeek score 43.10

Primer available yes

Label trnICAU-ycf2

Feature Metadata

label_name: trnICAU-ycf2
feature_type: igs
region: IRb
length_bp: 584
variable_sites: 10
pi: 0.00821
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnICAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	AACGCAGGACACTCCAAATT	50.0	45.0	58.0	58.0	678-826 bp	1.0	86.1
trnICAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	GCAGGAGCGAAACAATCAAC	50.0	50.0	58.0	58.0	879-1027 bp	1.0	85.5
trnICAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	AGGAAGATCCAGAAAGGCCT	50.0	50.0	58.0	58.0	1197-1345 bp	1.0	85.2
trnICAU-ycf2_p4	CACTTGACTGTCTACGGCTC	AACGCAGGACACTCCAAATT	55.0	45.0	58.0	58.0	1274-1422 bp	1.0	85.1
trnICAU-ycf2_p5	CACTTGACTGTCTACGGCTC	AGGAAGATCCAGAAAGGCCT	55.0	50.0	58.0	58.0	1793-1941 bp	1.0	84.7