Hotspot

ycf4-cemA

LSC · 65078-65949 · 872 bp

Back to Oenothera

Pi 0.00109

MarkerSeek score 50.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 3
pi: 0.00109
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCTTGACGCGTAGTGATGAT	CCGAGTGCGTAGTATTCCAC	50.0	55.0	57.8	58.2	1043-1059 bp	1.0	79.0
ycf4-cemA_p2	CCTTGACGCGTAGTGATGAT	CGAGTGCGTAGTATTCCACC	50.0	55.0	57.8	58.2	1042-1058 bp	1.0	79.0
ycf4-cemA_p3	CCTTGACGCGTAGTGATGAT	TCCGAGTGCGTAGTATTCCA	50.0	50.0	57.8	58.2	1044-1060 bp	1.0	78.3
ycf4-cemA_p4	TATTTCTTGCGCGTACCCAT	CCGAGTGCGTAGTATTCCAC	45.0	55.0	57.7	58.2	978-994 bp	1.0	78.2
ycf4-cemA_p5	TATTTCTTGCGCGTACCCAT	CGAGTGCGTAGTATTCCACC	45.0	55.0	57.7	58.2	977-993 bp	1.0	78.2