Hotspot

accD-psaI

LSC · 63393-64009 · 617 bp

Back to Oenothera

Pi 0.00541

MarkerSeek score 46.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 10
pi: 0.00541
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGCAATCCTTTAAAGGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.1	58.0	740-770 bp	1.0	75.0
accD-psaI_p2	CCACGCAATCCTTTAAAGGG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.1	57.9	746-776 bp	1.0	74.4
accD-psaI_p3	CGATCAAATTGTACCACGCA	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.8	58.0	753-783 bp	1.0	73.1
accD-psaI_p4	CGATCAAATTGTACCACGCA	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.8	57.9	759-789 bp	1.0	72.5
accD-psaI_p5	TCGATCAAATTGTACCACGCA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.3	58.0	754-784 bp	1.0	72.0