Hotspot

psbI-psbK

LSC · 58162-58547 · 386 bp

Back to Oenothera

Pi 0.00235

MarkerSeek score 48.80

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 3
pi: 0.00235
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	GGATTACGTCCTGGATCGTT	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	57.1	57.9	491-512 bp	1.0	76.9
psbI-psbK_p2	GGATTACGTCCTGGATCGTT	CCAAATTACCCGAGGCCTAC	50.0	55.0	57.1	58.0	577-598 bp	1.0	76.8
psbI-psbK_p3	GGATTACGTCCTGGATCGTT	TTAGCCAAATTACCCGAGGC	50.0	50.0	57.1	58.0	581-602 bp	1.0	76.7
psbI-psbK_p4	TCTTCACGTCCAGGATTACG	CCAAATTACCCGAGGCCTAC	50.0	55.0	57.1	58.0	589-610 bp	1.0	76.4
psbI-psbK_p5	TCTTCACGTCCAGGATTACG	TTAGCCAAATTACCCGAGGC	50.0	50.0	57.1	58.0	593-614 bp	1.0	76.3