Hotspot

trnKUUU-rps16

LSC · 4109-4866 · 758 bp

Back to Oenothera

Pi 0.00352

MarkerSeek score 51.60

Primer available yes

Label trnKUUU-rps16

Feature Metadata

label_name: trnKUUU-rps16
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 8
pi: 0.00352
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnKUUU-rps16_p1	ACGCACTTAAAAGCCGAGTA	CTTGAAAAAGGCGCTCAACC	45.0	50.0	57.6	58.2	892-963 bp	1.0	76.5
trnKUUU-rps16_p2	AGTCGTGGTCTTACAAACCC	CTTGAAAAAGGCGCTCAACC	50.0	50.0	57.5	58.2	966-1037 bp	1.0	75.6
trnKUUU-rps16_p3	GTCGTGGTCTTACAAACCCT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	57.5	58.2	965-1036 bp	1.0	75.6
trnKUUU-rps16_p4	AGACGCACTTAAAAGCCGAG	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.6	58.2	894-965 bp	1.0	75.5
trnKUUU-rps16_p5	ACGCACTTAAAAGCCGAGTA	AAGCGGGGGTTTTTACAGAA	45.0	45.0	57.6	57.6	843-914 bp	1.0	75.5