Hotspot

ycf1

SSC · 124677-130133 · 5457 bp

Back to Oenanthe

Pi 0.00819

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5457
variable_sites: 126
pi: 0.00819
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTCGTTTCAGAACTTGGA	CCTTGGTTCTGTTTCCCCAA	42.9	50.0	58.1	57.9	972 bp	1.0	74.5
ycf1_p2	TGCTTCGTTTCAGAACTTGGA	AAAAATCTCGGAAGCCTGGG	42.9	50.0	58.1	58.2	1380 bp	1.0	73.1
ycf1_p3	TGCTTCGTTTCAGAACTTGGA	GCCTGGGATTTCGTTCCATA	42.9	50.0	58.1	57.7	1367 bp	1.0	71.7
ycf1_p4	TGCTTCGTTTCAGAACTTGGA	ACGCAAACAAACCTTACCCT	42.9	45.0	58.1	57.9	989 bp	0.857143	68.6
ycf1_p5	CCAGTTTTTCCATTGCTTCGT	ACGCAAACAAACCTTACCCT	42.9	45.0	57.6	57.9	1002 bp	0.857143	66.3