Hotspot

petB

LSC · 75207-76612 · 1406 bp

Back to Oenanthe

Pi 0.00894

MarkerSeek score 45.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1406
variable_sites: 34
pi: 0.00894
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AACAGCTCCTTTGATGGGTG	TTCCAATGTAATGCGGCAGA	50.0	45.0	58.1	57.9	1915-1954 bp	1.0	80.0
petB_p2	AGCCCATTCTATGTTGGCAG	TTCCAATGTAATGCGGCAGA	50.0	45.0	57.9	57.9	1741-1780 bp	0.857143	74.6
petB_p3	AGCCCATTCTATGTTGGCAG	GCGGCAGATAGATAAAGGCA	50.0	50.0	57.9	57.8	1729-1768 bp	0.857143	74.2
petB_p4	AGCCCATTCTATGTTGGCAG	TGCGGCAGATAGATAAAGGC	50.0	50.0	57.9	57.8	1730-1769 bp	0.857143	74.2
petB_p5	AGCCCATTCTATGTTGGCAG	AACTTATGCGGTGGTTGGAG	50.0	50.0	57.9	58.2	1783-1822 bp	0.857143	74.1